Problem with directional cloning into pET101/D-TOPO, any advice?

Dear colleaques I have a problem with cloning of my PCR product (829 bp) into pET101 TOPO vector (5753 bp) by Invitrogen™ Champion™ pET101 Directional TOPO™ Expression Kit. I performed a PCR...

16 November 2023 9,845 4 View