How to concentrate proteins using Sephadex G-25 and dialysis?

The situation is as follows: Membrane protein elutes in acceptable purity after IMAC. pH is 7.5, 300 mM NaCl, 10% Glycerin, 0.1% DDM The problem is: Imidazole needs to be removed and buffer...

08 August 2013 4,059 18 View