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If I place a single copy of tGFP into the chromosome of my host under a strong constitutive promoter, will it produce enough tGFP to make the bacterial cell fluoresce? I know one copy of...
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I am working with a low copy plasmid that is AmpR. I want to shy away from the typical 100microg/mL. Is 20 microg/mL to low? 5 microg/mL?
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