Patricia Cathryn Swart

2 Questions 1 Answers 0 Followers

Questions related from Patricia Cathryn Swart

Has anyone successfully used pro BDNF (5H8) (sc-65514) to detect BDNF in the rat brain by Western Blot?

I am getting very faint bands and cannot seem to increase the signal. I have tried increasing the amount of protein per well, increasing the primary antibody concentration, 0.45 and 0.2 um...

04 April 2017 4,367 5 View

Extracting bacterial DNA from stool samples collected and stored (-80 freezer) in 95% ethanol

I am looking for any suggestions on how to extract bacterial DNA from stool samples collected and stored in 95% ethanol. (The extracted DNA will be used for 16S rRNA sequencing) - Kits used? -...

01 January 1970 1,923 2 View