Is neccesary to add methionine after BamH I cloning site in a pRSET A vector in orther to make the expression work?

Hello, I am trying to express a fusion protein using a pRSET A vector in C41(DE3) E. coli strains. I have tried many ways and I am trying to see if my sequence is correct. My question is if it is...

14 January 2020 8,036 2 View