Patricia Sampedro Piquero

3 Questions 9 Answers 0 Followers

Questions related from Patricia Sampedro Piquero

How can I prevent wave-like formations in brain sections embedded in paraffin?

I do not know if a product exists that I can add at the beginning of the immunohistrochemistry process to avoid the different solutions entering under my brain sections and producing this wave effect.

24 September 2014 4,493 7 View

With sections embedded in paraffin, is it possible to do immunofluorescence? Would you obtain a good resolution of a double-labeling?

I have several brain sections embedded in paraffin and I want to do double-labeling with immunofluorescence but I do not know if the resolution of the staining will be correct for the count.

05 September 2014 6,383 13 View

I am going to stain rat brain microglia with Iba-1 and I need to find the best protocol.

The rats (20 months-old) were deeply anesthetized with sodium pentobarbital and transcardially perfused with 4% paraformaldehyde. The brains were included in paraphin and cut in a microtome at 20...

09 March 2013 8,942 6 View