Patricia Rubio Reyes

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Questions related from Patricia Rubio Reyes

Have you cloned a PCR product containing 2X2A sequences into a vector that contains another 2A sequence?

Hi! I am having problems cloning a 1.6 kb fragment containing 2 different genes and 2X2A peptides into a vector that I already used to clone other fragments (digested with PmeI/BamHI). The vector...

18 December 2020 5,395 3 View

Hi! has anyone used Bovine or Avian PPD from Lelystad (Prionics)? Which concentration do you use for splenocyte restimulation after BCG vaccination?

I have used PPDB before from a different manufacturer but this new one comes in IU/mL, not in mg or ug, so I don't know how much to use.

30 August 2016 7,583 2 View