Patricia Cano-Sánchez

2 Questions 10 Answers 0 Followers

Questions related from Patricia Cano-Sánchez

Which is a suitable system to obtain recombinant proteins that contains disulfide bridges in E. coli?

Hello everybody, I have been expressing proteins that contain disulfide bridges and I usually use pET32 which cantains Trx as chaperone and Rosetta gami as strain. This system works for some...

06 April 2015 5,658 14 View

Why nikel ion does not bind to Ni-NTA column after APS treatment?

I treated Ni-NTA column with 10% APS because I purified a protein using a buffer containing DTT and it tourned brown. After APS treatment I wash the column with water and then I stripped it with...

11 July 2014 3,640 7 View