Pascal Mirleau

2 Questions 7 Answers 0 Followers

Questions related from Pascal Mirleau

Are there any PCR methods and tricks when using proofreading pfu DNA polymerase and 50bp primers for Metabarcoding ?

To avoid frame reading errors during PCR amplification with conventional Taq polymerase, we use pfu DNA polymerase. However, testing several annealing temperature with 50bp primers only allowed...

12 December 2014 4,943 4 View

Metabarcoding project : can you recommend particular primers and references targetting 18S rDNA?

I am studying the diversity of organisms colonizing seagrass leaf surface and wish to target eukaryotic SSU, among other markers, using a metabarcoding approach.

01 January 1970 8,214 7 View