Parvin Most

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Questions related from Parvin Most

Protein localization problem

Dear all, I have a protein which full length contain 146 amino acid, and its first 14 amino acid is predicted as nuclear localization signal. After excluding 14 amino acid, I found it is localized...

22 November 2016 7,472 13 View

What are the possible reasons for my cloning ligation failure from pGEMT to plant expression vector using the retriction enzymes Kpn1 and SalI?

Hi Everybody, For last 2 month I continuously failed to ligate my 399 bp into 12000 bp vector. The insert was cloned into pGEMT vector. I tried with different controls, there is no colony at...

22 July 2016 5,405 13 View