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I am doing plaque assay for CHIK-V virus. After incubation plate was wash fix with 3.7% formaldehyde sol. After fixing cell was detected. Before fixing monolayer was fine.
23 June 2021 9,048 6 View
Initially, the sample was giving hemagglutination with chicken RBC, but after passages in Frey's & PPLO w/o cv broth medium without l-cystine HCl, there is no hemagglutination with chicken RBC.
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