Parvathi Suma

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Questions related from Parvathi Suma

Why do we keep it at 95 degrees for 15 secs in pcr and why not another time?

In a PCR After the initialistion step at 95 degrees for 1-2 mins, in the second round my guide kept for 15s again at 95 degrees. I know that it is a step in the thermocycle. But why did he keep...

07 July 2014 7,558 16 View

Does anyone have any knowledge on gel extraction and DNA purification?

During DNA gel purification using a centrifugation technique, I accidentally centrifuged the sample at 1300 rpm instead of 13,000 rpm. What will be the consequence? The gel along with DNA weighed...

07 July 2014 2,088 2 View