Paromita Saikia

3 Questions 6 Answers 0 Followers

Questions related from Paromita Saikia

Problem in restriction based cloning. How to replace GUS site in pCAMBIA 1301?

I am trying to clone my targeted gene by replacing GUS site of pCAMBIA1301 to utilize the 35S promoter. For that I have used 2 restriction enzyme i.e. NcoI and BstEII, and both the enzymes are...

02 July 2024 10,000 7 View

What should be the PCR profile of full length gene amplification with amplicon size more than 1.5 kb?

I am facing problem to amplify full length gene (around 1.5 kb) using cDNA with primer length varying from 24-28 decamer( attached with adapter) at tm 62 degree celsius. What PCR profile I should...

08 December 2023 8,255 3 View

What is the procedure to add 2 restriction sites to primers for cloning a complete gene sequence and is it necessary to add setting sequence ?

how to add two restriction enzyme sites to primers for cloning a gene sequence (size 1-2kb) to pGEM-T Easy Vector followed by pcambia 1301 and is it necessary to add setting sequence before the...

21 September 2023 7,515 4 View