Paromita Saikia

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Questions related from Paromita Saikia

What should be the PCR profile of full length gene amplification with amplicon size more than 1.5 kb?

I am facing problem to amplify full length gene (around 1.5 kb) using cDNA with primer length varying from 24-28 decamer( attached with adapter) at tm 62 degree celsius. What PCR profile I should...

08 December 2023 8,166 3 View

What is the procedure to add 2 restriction sites to primers for cloning a complete gene sequence and is it necessary to add setting sequence ?

how to add two restriction enzyme sites to primers for cloning a gene sequence (size 1-2kb) to pGEM-T Easy Vector followed by pcambia 1301 and is it necessary to add setting sequence before the...

21 September 2023 7,418 4 View