4 Questions 3 Answers 0 Followers
Questions related from Parisa Moghaddam-Taaheri
Hi, I'm having an issue with cloning the full length of my PCR product into my vector. What I have done: 1.) PCR to amplify my insert where primers add 5' NheI and 3' BamHI sites (with 6 extra...
17 April 2017 6,502 6 View
Some DMSO (dimethyl sulfoxide) was spilled on the plastic part of our thermal cycler and when a cycle was ran, it corroded the plastic from the lid to that of the base of the instrument such that...
10 April 2017 3,307 4 View
To avoid an additional cloning step, I want to use whole plasmid PCR. I have designed my primers back to back (i.e. non-overlapping) and they are located in the origin of replication of my...
15 December 2015 1,835 3 View
I needed to do a culture and subculture all in one day but my cells are growing slowly. I'm growing BL21-DE3 cells transformed with plasmid and started a culture from a freeze into 3 mL LB+Amp for...
22 April 2015 7,123 4 View
