Pardis Prsh

3 Questions 5 Answers 0 Followers

Questions related from Pardis Prsh

Why a pair of primers give different melt curve in same DNA concentration ?

Hi everybody I'm trying to genotype two SNPs with HRM. one of them(SNP1) is set up but the other one(SNP2) shows different melt curves in DIFFERENT temperature(some samples in 92 C ,some in 86 C...

21 February 2019 216 2 View

How can I analysis the results of HRM genotyping?

I tried to detect two SNP with rotor gene 6000, and after I get melt curve, I don't know how I should fix it. The graph I get is not like what I have seen in the articles, and I think I have to...

02 November 2018 4,593 4 View

Wich HRM master mix is better between Norgen, Amplicon and Solis BioDyne?

I want to detect SNPs with HRM method.

08 June 2018 9,259 1 View