Paras Gaur

2 Questions 4 Answers 0 Followers

Questions related from Paras Gaur

How can I elute my protein from NI NTA beads, in presence of 6M GuHCl (Guanidium Hydrochloride) ?

I am doing whole purification in 6M GuHCl, but I am not able to elute the protein with 500mM Imidazole. Is there any precipitation of Imidazole in presence of GuHCl ?

05 May 2016 932 4 View

How to elute proteins from Ni Beads in denaturating conditions ?

I am trying to purify a His-tagged protein in denaturating conditions (Guanidium Hydrochloride). I can see the protein on beads and in cell lysate but I am not able to see anything in my elutions....

03 March 2016 3,663 7 View