Why my Twin-Strep tagged protein is not eluting out from Strep-Tactin® Superflow® high capacity resin?

Hi, I am using Strep-Tactin® Superflow® high capacity resin by IBA to purify a Twin-Strep tagged fusion protein at N-terminal. I am facing issue to elute out the protein. For elution I have...

31 December 2023 5,218 1 View