Paola Andrea Martinez Murillo

3 Questions 6 Answers 0 Followers

Questions related from Paola Andrea Martinez Murillo

Human Luminex how to resolve low values if last standard point works for some plates and not for others?

Hi, I have run 16-plex and for some markers and in some plates the standard curve was not clear for the last standard dilution, but in other plates all points worked. I'm interested in calculated...

03 March 2020 9,691 1 View

Is it normal to find two big populations when you exclude doublets in flow cytometry (FSC-H vs FSC-W)?

I've been using MoFlo xdp to sort bone marrow samples to isolate plasma cells... When I exclude the doublets there is always one big singlet population... But last time I got two big populations...

09 September 2013 8,550 8 View

Can I use both cells and beads for the compensation?

In the lab we have beads for the compensation, but I've tried them and two antibodies don't bind those beads. Can I use both cells and beads for the compensation? I was thinking to use the...

09 September 2012 6,581 6 View