Which could be the cause for fluorescence/autofluorescence in APC-channel in a flow cytometry experiment?

I had a problem with flow cytometry. I did a cultive with PBMCs in RPMI 10% Bovine Fetal serum, 1% glutamine, 1% streptomicine/peniciline, for 24 hours, 37ªC, 5% CO2. Then I stimulated the cells...

20 September 2018 3,284 5 View