Pankti Parikh

2 Questions 2 Answers 0 Followers

Questions related from Pankti Parikh

Does anybody use Trp promoter/operator based expression vector?

Hello Everyone, Does anybody use a Trp based expression vector? I had a few questions. 1. Are there any such vectors commercially available? If yes, please suggest the companies selling them. 2....

14 March 2017 6,416 2 View

Single site cloning - Any enzyme to blunt one end to avoid self ligation?

I am trying to clone a small gene (180bp) in to a ~6kb vector, using XhoI site only. I've been treating the vector with alkaline phosphatase (Roche). However, I'm getting colonies having self...

11 November 2016 3,427 10 View