Panini Patankar

2 Questions 3 Answers 0 Followers

Questions related from Panini Patankar

How to separate microglia from mixed neonatal rat (Day 2) cortical culture?

For my project, I have been trying to separate microglia from mixed culture using shaking method but have not yet been successful. Astrocytes manage to creep in after shaking and ultimately over...

14 July 2025 9,218 4 View

Is it wise to add phosphatase inhibitors to the PFA while doing transcardiac perfusion in rats for detection of phosphoproteins using IHC?

I was trying to visualize phospho-ERK and phospho-Cofilin in rat brain sections. However, the images were disappointing and after doing some literature search, I realized that this may be due to...

08 April 2024 3,984 0 View