Pachineella Lakshmana Rao

5 Questions 8 Answers 0 Followers

Questions related from Pachineella Lakshmana Rao

Bigger size plasmids restriction digestion, approximatly 24 kb?

I have cloned 9 fragments in the PCIneo vector, I have inserted the 10th fragment in it. In colony PCR it is showing exact band size, but in restriction digestion, I could not able to see...

12 April 2021 5,729 8 View

Plz anyone help, one of my crystallisation condition is 20%peg3350, 5%yPGA and 0.1 M sodium acetate ph 4.5 not increase in size and diffraction?

i have tried seeding and with salts but there is not much increasing in size eventhough they are small we have tried diffraction but it is not diffracting, the gud news that they are protein...

05 December 2016 4,522 4 View

Protein Dimer modeling?

i have protein dimer sequences of chain A and B, how can i model these two sequences? i want to get as dimer. is there any software ? can you suggest?

19 October 2016 9,711 1 View

Molecular dynamics and simulation?

Basically how many nanoseconds we have to use for protein simulation? i used 20 ns for modeled structure, is it sufficient or shall i increase the time? can you suggest anyone?

08 September 2016 9,697 7 View

Protein-ligand simulation?

i have modelled one protein by using modeller, after docking protein with suitable ligand, it is interacting with five amino acid residues, then i submitted that complex to 20 ns simulation...

01 September 2016 9,782 3 View