Pachineella Lakshmana Rao

4 Questions 7 Answers 0 Followers

Questions related from Pachineella Lakshmana Rao

Bigger size plasmids restriction digestion, approximatly 24 kb?

I have cloned 9 fragments in the PCIneo vector, I have inserted the 10th fragment in it. In colony PCR it is showing exact band size, but in restriction digestion, I could not able to see...

12 April 2021 5,694 8 View

Protein Dimer modeling?

i have protein dimer sequences of chain A and B, how can i model these two sequences? i want to get as dimer. is there any software ? can you suggest?

19 October 2016 9,669 1 View

Molecular dynamics and simulation?

Basically how many nanoseconds we have to use for protein simulation? i used 20 ns for modeled structure, is it sufficient or shall i increase the time? can you suggest anyone?

08 September 2016 9,663 7 View

Protein-ligand simulation?

i have modelled one protein by using modeller, after docking protein with suitable ligand, it is interacting with five amino acid residues, then i submitted that complex to 20 ns simulation...

01 September 2016 9,750 3 View