Pablo Andrés Muñoz Llancao

4 Questions 19 Answers 0 Followers

Questions related from Pablo Andrés Muñoz Llancao

How to calculate standard and sample concentration on competitive ELISA?

Hello, I am trying to do the Competitive cAMP Complete ELISA Kit from ABCAM. we are following the instruction of the manual but is not very clear in how to make the calculation. We would like to...

30 September 2018 3,191 6 View

How to avoid donor bleach (CFP) in a FRET acceptor photobleaching experiment?

I am doing a FRET acceptor photobleaching experiment with a CFP-YFP biosensor pair. The problem is that I am having bleach of the CFP donor fluorophore. I am trying differents protocols from...

09 November 2016 5,020 9 View

Is there any problem if I transfected a FRET sensor (CFP-YFP) and a GFP plasmid?

I want to transfect a shRNA-GFP against my protein of interest and observed how much this loss of function will affect the activity of other protein with a FRET ratiometric biosensor...

18 October 2016 1,662 2 View

Why is the effect of a constituvely active protein different for the pharmacological activation?

The use of a pharmacological agonist of a protein induces morphological changes in the neuron, the constituvely active form of the protein does the same, however the effect seems high with...

02 December 2015 1,126 3 View