P Lokamitra P Lokamitra

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Has anyone here done CCK-8 assay here with suspension cells ?

I am trying to find out how much cells are viable after my incubation. I am having issues with working out the standard curve. Seed the cells in 96 well plate 'v' bottom and transfer to a flat...

18 January 2022 8,072 7 View

What is the best way to avoid loosing cells (suspension cells) when washing with PBS ?

I am culturing suspension cells (Jurkats) and they are in 96 well plate ('v' bottom). I have been noticing a lot of cells (>50%) being lost despite centrifuging them and aspirating the media from...

17 January 2022 7,999 6 View