Ozge Uysal Yoca

2 Questions 7 Answers 0 Followers

Questions related from Ozge Uysal Yoca

I have q question about TOPO TA cloning. I have a PCR Product that Platinum Taq DNA polymerase is used. What should I do?

I didn't design my primers that produce A overhang. And I have to do cloning. I read that I can use dATP and nonproofreading Taq to produce A overhang. Can I use this method to add an A to my PCR...

02 June 2023 3,833 3 View

We tried to DNA isolation from FFPE tissue by manuel method but we have some problems in PCR. There are so many smear in agorose gel. Help pls?

We isolated DNA from FFPE by manuel method. We done PCR but in gel there are some smear and we dont know how can we solve this problem. Can anyone have a solution?And why saw smear in PCR?

28 May 2018 578 12 View