Ouzaouit Abdelhak

3 Questions 5 Answers 0 Followers

Questions related from Ouzaouit Abdelhak

How can I make high-quality SDS-PAGE gel and improve the gel's appearance taking detail into consideration?

I am using a self made Bis-Tris gel in combination with MOPS buffer. The attached picture shows a 1.5 mm comb with 10 wells. I am loading 20 µL of the sample and 8 µL of the protein ladder (10–235...

04 August 2025 9,078 4 View

Why can't I successfully express my protein in the BL21(DE3) host cell? It seems there is no purification happening also?

I am trying to express the Nephrin protein (115 kDa) after obtaining the full-length extracellular domain of the gene of interest (GOI) in the pET28a(+) plasmid, which contains the T7 promoter. I...

14 March 2025 9,606 4 View

Does anyone know what cause the U-shape of bands and if you can provide all instructions, gel percentage.. i wll be thankful ?

my Q related to electrophoresis gel is; we run a gel on 80v per 30min, product size is between 1000bp and 2000bp,

29 June 2024 3,798 2 View