21kDa target protein not observed in 30k MWCO amicon centrifugal filtrate post cleavage?

Hello all, I expressed my fusion protein bound to MBP using a pMAL-c2e vector and purified it using an amylose column. Upon running a cleavage reaction of 1 mg of my fusion protein in 1 mL of 20...

17 March 2025 6,915 0 View