I always get false positives when I do lambda red recombination. What could be the reason for this?

I want to insert the km resistance gene into the ampc gene in E.coli. I want to do this with Datsenko and Wanner method. I amplify the km gene from the pkd4 plasmid with a 40 base tail at the...

21 March 2023 5,854 1 View