Oskan Tasinov

3 Questions 18 Answers 0 Followers

Questions related from Oskan Tasinov

Could someone suggest a cell culture model for analysis of glucose uptake?

I am planning to analyze the glucose uptake in 3T3-L1 cells. The suggested treatment scheme will be used to analyse the effect of plant extract on glucose uptake. Control groups...

16 October 2013 9,737 7 View

Cultivation of human PBMC? Protocol for treatment of PBMC's with LPS?

1. Could someone recommend a culture media for human PBMC cultivation? 2. What concentration of LPS should I use to induce inflammation in cultured PBMC? I found that concentrations between...

01 April 2013 8,943 20 View

I need to isolate the peripheral blood mono-nuclear cells called buffy coat (PBMC/MNC) from blood using Ficoll. Is there any protocol?

I want to separate peripheral blood to its components (erythrocytes, leukocytes etc.) using Ficoll. Possibly ıts density gradient centrifugation needed, but I can't find a protocol for separation...

09 March 2012 8,337 14 View