Oscar Kai

4 Questions 4 Answers 0 Followers

Questions related from Oscar Kai

How can one estimate Retics in whole blood using Flow cytometer without staining for White Cells

I have been reading the BD Retic Count protocol and it seems it does not take into account the white cells. Is the assumption that their presence in relation to rbc is very minimal that in the end...

10 October 2013 3,274 1 View

Is there a substitute in use of Ethidium Bromide in studying malaria infected red cells?

I remember we had been using Acridine orange as a nucleic acid dye in malaria studies. I cannot remember when and especially why we switched to using EtBr as a nucleic acid dye in malaria...

07 July 2013 8,201 12 View

When do you use mean fluorescent intensity and when to use proportion?

During acquisition/analysis of flow data: what is the best practice? Can one use Mean Fluorecent Intensity (MnI) even when data has a nice dual population showing distinct negative and positive...

06 June 2013 2,564 4 View

Do other people experience a lot of clogging especially when using Cyan ADP cytometer?

And how do you go about cleaning or its maintenance to stop this?

05 May 2013 2,599 2 View