Oscar Garrett

2 Questions 2 Answers 0 Followers

Questions related from Oscar Garrett

How to clone a potentially toxic insert for rice transformation?

Hello fellow scientists! I'm currently trying to construct a vector for agrobacterium-mediated transformation into rice. Our construct aims to overexpress two proteins (protein A and protein B),...

15 September 2023 6,195 3 View

How to determine substrate / coenzyme concentrations for determination of inhibitor IC50, with the goal of comparing between two homologous enzymes?

I'm presently attempting to assay the activity of dihydroorotate dehydrogenase (DHODH) from two different species in the presence of a couple different inhibitors. For one DHODH, I already...

24 April 2023 4,624 1 View