Oscar Crespo-Salvador

4 Questions 15 Answers 0 Followers

Questions related from Oscar Crespo-Salvador

How to prepare aliquots of freezing medium (for PBMC)?

Hello everyone, I am working on a project where I receive daily samples to freeze at -80 °C. Specifically they are peripheral blood mononuclear cell (PBMC), until now I was using CryoStor® CS10,...

23 April 2024 3,886 6 View

How to prevent primer pairs with "Products on potentially unintended templates" from appearing on Primer-BLAST?

Hello everyone, I am designing some primers with Primer-BLAST and this problem keeps happening to me: I specify the characteristics of the primers, the amplicon, the number of pairs, the...

31 May 2021 7,701 3 View

Can someone assist me with a problem with DNA extraction in ChIP protocol?

Hello, I am performing ChIPqPCR with plants as I explained in another...

21 September 2015 7,867 14 View

Am I adding too much chromatin if I get no signal differences in my ChIPqPCR ?

Hello, in my PhD thesis I have to use ChIPqPCR to study the differences in plant's chromatin (control vs infected). To do so, I use some antibodys that bound to widely recognised histone...

24 August 2015 4,538 24 View