Oron Berkowitz

2 Questions 2 Answers 0 Followers

Questions related from Oron Berkowitz

What should be the "my plasmid"/GFP plasmid ratio in co-transfection?

Hi, I would like to co-transfect U2OS cells with GFP-containing plasmid, and my plasmid of interest using Polyjet transfection reagent. The main idea is to measure trasnfection efficiency, and...

18 March 2017 6,367 1 View

Cloudiness after adding isopropanol in RNA isolation using TRI-reagent. Why does it happen?

HI. I isolated RNA many times, and suddenly something weird happened: I used TRI-reagent 0.5 ml, and stored the tubes on the tubes rotator for 10 min. I added 50 uL BCP, waited 5 min, and...

23 February 2017 2,228 5 View