Orli Thau-Zuchman

4 Questions 3 Answers 0 Followers

Questions related from Orli Thau-Zuchman

IHC on organotypic slice culture

I am due to start IHC on cerebellar organotypic slice culture (DIV21), and have some questions: 1. What is the recommended time frame for fixation? I saw, in different protocols, a range between...

02 February 2016 6,254 4 View

How can I make sure the cerebellar organotypic slices are alive?

I am doing cerebellar organotypic slice culture from mice p8 and would like to know if there is a way of making sure the slices are happy and alive? I would also like to know if it is normal to...

02 February 2016 7,917 4 View

Can anyone suggest a systematic characterization method for microglial cells (for different activation stages) in TBI mouse?

I have done a fluorescent IHC-P for Iba-1, and tried to double stain it with CD16/32 (the second Ab seems to failed). Iba-1 is a good staining but too general.

02 February 2014 7,693 2 View

What is the best way (or software?) to count cells after fluorescent and DAB IHC staining?

02 February 2014 8,315 2 View