Oriana Mazzitelli

3 Questions 6 Answers 0 Followers

Questions related from Oriana Mazzitelli

Can anyone suggest me a suitable releasing agent during magnetic selection of cells?

Hi I am using Dynabeads Expander and I am loosing most of my cells during removal of the magnetic beads. It seems as though most cells are remaining adhered to the beads so when I remove the beads...

24 January 2016 6,246 12 View

What is concentration of IFN-gamma used to test effect on CD4+ cells?

Hi I am working with CD4+ cells and I am trying to investigate the effect of IFN gamma and how it effects differentiation of these cells. What are the quantities in Units/ml that of IFN gamma...

11 January 2016 9,776 2 View

What is the total volume of buffy coat needed to obtain 1x10^8 lymphocytes?

The kit I am using states that I need to start off with 1x10^8 of lymphocytes isolated from buffy coats. Does anyone know the total volume of buffy coat I should start off with in order to obtain...

15 September 2015 3,852 4 View