Ong Qian Wei

6 Questions 6 Answers 0 Followers

Questions related from Ong Qian Wei

Why is there growth in my Bacillus subtilis negative control plate 2 days after transformation?

Hi, I have recently attempted natural competence transformation with Bacillus subtilis (WB800N), following the protocol from Mobitec. I used 5ug/ml chloramphenicol for my selection. My...

25 June 2022 9,046 0 View

RAW 264.7 nitric oxide assay with griess reagent, do you refresh media before treating the cells?

Hi, This will be my first time doing this assay. For context, my experiment will be to determine if some compounds have any anti-inflammatory effect. I plan to do the following steps: 1) Seed...

26 May 2022 526 3 View

Does Bacillus subtilis WB800N strain have some level of kanamycin resistance?

Hi, I just started using this strain of B.subtilis, and during transformation (Heat shock, natural competence), I noticed the negative and positive control plates looked exactly the same (I am...

25 February 2022 1,789 3 View

Can I autoclave 100% glycerol?

Can you autoclave 100% glycerol straight up to sterilize it? Will it result in caramelization or will it be fine? I am aware I can dissolve it in water and autoclave it but it would make things...

16 February 2022 4,473 2 View

Will 10x cutsmart buffer affect restriction enzyme?

For context, I am doing multiple restriction digestions (>50) and I want to streamline it by making a 10x cutsmart buffer + restriction enzyme mastermix. Reaction volume will be 50ul so the...

18 October 2021 1,097 4 View

What could have caused this result on the gel (SDS PAGE)?

SDS PAGE experiment had a weird result. Are there any ideas as to why the gel turned out like this? Could it be that the gel was very dense or the acrylamide mixture was poured unevenly resulting...

01 January 2018 2,588 4 View