Omer Shomrat

4 Questions 7 Answers 0 Followers

Questions related from Omer Shomrat

How can I remove mucus from mouse intestine without removing the epithelium?

Hey there, I've recently worked on a method for intestinal epithelial cell (IEC) isolation and digestion into single cells (followed by flow cytometry) that is very satisfactory (for my needs),...

14 May 2021 5,961 3 View

QPCR: what could be the reason for much higher CTs for housekeeping genes in one sample but not the others within the same group?

Hey there, I wonder if anyone can help me with the following issue: I’ve been running recently qPCR reactions on cDNA prepared from intestinal epithelium-enriched samples and ran into a recurring...

17 May 2020 4,085 1 View

How can I stimulate T cells to proliferate in a completely TCR-independent fashion?

As part of an effort to determine whether a certain proliferative response of virtual/innate memory T cells (CD44hi) I tried to knock-out the TCRa of these primary T cells and to culture them with...

18 February 2017 5,822 9 View

How shall I extract all the proteins from a cell line?

Hey everyone, I have a cell line suspension which causes T cell activation in what seems to be an antigen-specific manner, and I would like to find out the antigen. In short, I'm thinking of...

25 December 2016 5,635 9 View