Omar Wehbe Al Masri

2 Questions 4 Answers 0 Followers

Questions related from Omar Wehbe Al Masri

How can the limit of detection in competitive lateral flow immunoassays (LFIA) be changed?

I'm trying to change the limit of detection (L.O.D) of my target analyte for an LFIA (competitive) test strip. So far, I have attempted to change both the volume of my conjugate antibodies on the...

01 January 2020 5,270 3 View

Phage display (Ph.D7): What are the consequences of eluting bound phage with glycine-HCl buffer (pH 2.2) for 35 minutes instead of 10-20 minutes?

On day 6 (final day) of the phage display procedure, I allowed the bound phage to elute with gentle rocking using glycine-HCl (pH 2.2) for 35 minutes instead of the recommended 10-20 minutes and...

07 July 2018 3,472 5 View