Omar Santín Martínez

4 Questions 18 Answers 0 Followers

Questions related from Omar Santín Martínez

Can Sepharose-Nanobody-(GFP) columns be regenerated after a GFP-tagged protein purification?

I am going to purify several proteins by using GFP-nanobodies linked to a sepharose column since my proteins have a GFP taq. Since I have not experience with this protocol, I would like to solve...

11 November 2018 6,194 1 View

Does all the Baculovirus Bac-to-Bac-like systems share donor plasmid compatibility?

I am going to clone several protein to express in Baculovirus. I am clonning them using one plasmid that I know is compatible with the Bac-to-Bac system that is being used in the lab, (since is...

10 October 2018 8,100 1 View

Why urea is removed from urea-acrylamide gels before running?

This is a very basic question that I have that makes me think that I do not fully understand the basics of why we add urea to the gels. I have tried to look in the literature but I did not find...

08 August 2017 3,391 3 View

Protein Km comparation

Basically I have now two proteins that catalyze the same reaction, but with different affinity for their substrate. The first protein has a lower Km since is enough to visualize the reaction...

06 June 2017 4,003 8 View