Om Prakash Chouhan

3 Questions 14 Answers 0 Followers

Questions related from Om Prakash Chouhan

How i can separate GTP (Guanosine triphosphate) and c-di-GMP molecule by HPLC method ??

i am trying to separate GTP (Guanosine triphosphate) and c-di-GMP molecule by HPLC using Water's Xterra RP-C18 column (4.6X250mm). i used isocratic or gradient both solvent system of water and...

03 March 2016 4,323 4 View

How can I do protein purification by affinity chromatography from a cut protein tag?

I am trying to purify my recombinant GST fusion protein by affinity chromatography, after chromatography I want to remove GST tag from protein so I did in column digestion with protease after...

09 September 2014 3,775 24 View

How can I express and purify my desired protein by affinity?

I did cloning after site directed mutagenesis and i got a cloned cell. It is supposed to be 62 kd but i am getting around 52 kd. I don't know what the problem is. Does anyone have any idea what's...

04 April 2014 6,580 11 View