Olumuyiwa Igbalajobi

2 Questions 8 Answers 0 Followers

Questions related from Olumuyiwa Igbalajobi

3.5kb cDNA amplification and cloning for sequencing?

I wish to amplify 3.5kb cDNA of a gene of interest and then clone into pjET vector for sequencing. I am having issues with the primer design. I intend to design forward primer at every 500 bp and...

10 October 2016 2,141 2 View

CRISPR Cas9 induced deletion?

Is it possible for the next ATG in frame after the deletion of the ORF ATG still translates to a functional protein?

01 January 1970 8,407 6 View