Olivia Matthews

2 Questions 3 Answers 0 Followers

Questions related from Olivia Matthews

What are the best conditions for transferring a >200kDa and ~40kDa protein?

I am having issues transferring my large protein (220kDa) at 30V for 2 hours (RT). It is often patchy with incomplete bands (annoyingly I occasionally I get a clear transfer!). For my other...

08 August 2019 8,333 7 View

Western blots on tissue which have been stored in RNAlater?

I have used up my snap frozen tissue from an experiment but have some left in RNAlater (invitrogen). I was wondering if anyone had any experience with running western blots using tissue that has...

08 August 2019 5,691 3 View