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Questions related from Olivia Harding
I am preparing to have single cell RNA seq done on mouse tissue and I am using a protocol for single cell suspension we use for flow cytometry: Resect tissue, mince, digest in buffer, lyse red...
19 September 2023 7,402 0 View
If the blocking period's purpose is to prevent non-specific binding of the primary antibody in the following step, why do many protocols use the detergent TWEEN in the blocking buffer? Don't...
21 May 2015 829 10 View
The protocol seems to be exclusively for nucleic acids in solution, but can the reagent be used as an immunofluorescent probe?
11 March 2015 4,459 7 View
It is a unidirectional, smooth drift of 1-3 microns in a 6 micron stack. I would also be interested in general ideas for coding this sort of program.
24 February 2015 8,989 1 View
