Olivia Harding

3 Questions 6 Answers 0 Followers

Questions related from Olivia Harding

Should I use DNase to prep single cell suspension for scRNA-seq?

I am preparing to have single cell RNA seq done on mouse tissue and I am using a protocol for single cell suspension we use for flow cytometry: Resect tissue, mince, digest in buffer, lyse red...

19 September 2023 7,345 0 View

What is the purpose of TWEEN in the blocking buffer?

If the blocking period's purpose is to prevent non-specific binding of the primary antibody in the following step, why do many protocols use the detergent TWEEN in the blocking buffer? Don't...

21 May 2015 746 10 View

Has anyone had experience with a reliable image registration program/plugin that adjusts for z-shift?

It is a unidirectional, smooth drift of 1-3 microns in a 6 micron stack. I would also be interested in general ideas for coding this sort of program.

24 February 2015 8,946 1 View