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Questions related from Olga Sizova
Hello, I need to detect mCherry+ cells in vitro using fluorescence microscope. I am sure mCherry+ cells are there, but mCherry fluorescence may be decreased. I have tried conjugated mCherry-PercP...
09 September 2017 6,857 4 View
Hello, could anyone provide me the information about p53 status of B16F1 mouse melanoma cancer cell line? Unfortunately, I fail to find any reference or information in the databases. Thank you!
10 October 2016 2,261 3 View
Hello, we have recently purchased B16F1 (mouse melanoma) from ATCC. At first cells grew ok (used:Dmem from Sigma +10%FBS), but the medium was getting darker starting form next they after seeding....
08 August 2016 3,999 5 View
Hello, For SDS-PAGE western blots: do you usually run your gels on ice or room temp.? For example if I use these conditions: Stacking 70V 30min, then separating 120V for 2 hrs. Is it okay to run...
07 July 2016 8,421 4 View
Hello everybody, I am trying to find a stable B-lymphoma cell line tagged with GFP. Can anyone please help me? It seems there is no cell line like this and people insert GFP by themselves. But the...
10 October 2015 3,582 2 View
