Olga Papadodima

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Questions related from Olga Papadodima

Which is the best method to remove PCR inhibitors, like melanin, from RNA preparations?

Hello everybody! We are trying to isolate DNA/RNA from thin primary FFPE melanomas. We use a protocol based on silica columns for the isolation of DNA/RNA from the same specimens. Although the DNA...

26 March 2021 8,235 3 View

Does ethanol affects the isolation of DNA from FFPE tissue sections?

Hello! During DNA extraction from FFPE samples by a kit using silica columns, the first step is the addition of xylene to deparaffinize the sample. Next, you add ethanol to the pellet. Does anyone...

30 July 2015 9,189 2 View