4 Questions 6 Answers 0 Followers
Questions related from Olga Kudryavtseva
I have a basic IHC question, there is Tris-HCl buffer in the protocol I had gotten. How exactly to make it? There is also neither molarity nor pH specifications...I've been told that usually it's...
08 August 2018 7,989 6 View
Hello everyone, I am doing membrane potential measurements wit sharp glass microelectrode (resistance 20 - 120 mOhm, typically 50 mOhm) on porcine perivascular retina in vitro and now I need to...
01 January 2018 8,127 6 View
Hello everyone, I am working with pericyte-like cells whic have long processes. I imagine Ca2+ in these cells using Oregon green and LSM800 laser scanning confocal micsroscopy. I add an agonist...
08 August 2015 1,449 3 View
It's the first time I have to optimize a DAB staining and I faced some difficulties. I am staining paraffin-embedded humna retinal tissue against NG2 protein, which should be expressed in...
03 March 2015 4,077 16 View
