Olga Kozhar

5 Questions 9 Answers 0 Followers

Questions related from Olga Kozhar

Why annotated and assembled transcriptomes produce different results with tagseq alignment?

I am working with tagseq data that I am aligning to a reference transcriptome of an organism. I used several approaches to produce a transcriptome: using Braker annotation pipeline (with reference...

29 September 2023 2,280 1 View

Why PCR primers stop working with the addition of MgCl2?

I have designed a pair of DNA primers to amplify 700bp region in a fungus. When I tested these primers they produce heterogeneous amplifications among different strains, in other words amplify...

17 November 2021 6,496 3 View

How to correctly choose population strata and interpret results for the detection of loci under selection from RADseq data using Bayescan?

I am working on the analysis of fungal populations sampled from 12 geographic locations using RADseq data. The dataset contains 7439 polymorphic loci with ~10 snps per RAD locus (75,482 snps...

20 May 2020 832 3 View

Minimum spanning network using microsatellite data?

I am looking for a way to build a haplotype network using SSR data. I have been using Poppr R package before, but I want to see the distance between isolates in nodes/steps. In Poppr the network...

14 November 2017 5,586 5 View

How do I solve Rhizopus contamination in Botrytis cultures?

Hello, we are isolating Botrytis cinerea from fresh fruit of berries and ran into the problem with contamination of our cultures with Rhizopus. It growth pretty fast on fresh fruit we incubate to...

28 October 2015 2,991 6 View