Olga Burenkova

8 Questions 19 Answers 0 Followers

Questions related from Olga Burenkova

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

06 August 2024 9,508 2 View

What is the easiest but still effective method for slides pre-cleaning before poly-lysine coating for IHC?

I see in the instructions and in some other manuals for poly-lysine coating that slides must be cleaned 'before attempting this procedure. Clean with acidic alcohol (i.e., 1% HCl in 70% ethanol)...

15 February 2024 8,111 4 View

Can poly-lysine be applied to charged slides?

We have a surplus of positively charged slides (Superfrost Plus Microscope Slides) since they did not perform well with small zebrafish brain slices, which were washed off from these slides during...

14 February 2024 2,660 4 View

What is the reason for the partial lack of focus in images of adult zebrafish brain slices?

I recently conducted staining on brain sections of adult zebrafish using Nissl stain. The brains underwent pre-fixation in 4% paraformaldehyde, followed by storage in 75% ethanol at -20°C for a...

13 February 2024 4,081 7 View

What is the reason for the partial lack of focus in images of adult zebrafish brain slices?

I recently conducted staining on brain sections of adult zebrafish using Nissl stain. The brains underwent pre-fixation in 4% paraformaldehyde, followed by storage in 75% ethanol at -20°C for a...

01 January 1970 2,096 8 View

Slides pre-cleaning before poly-lysine coating for IHC

I see in the instructions and in some other manuals for poly-lysine coating that slides must be cleaned 'before attempting this procedure. Clean with acidic alcohol (i.e., 1% HCl in 70% ethanol)...

01 January 1970 381 0 View

Reusing positively charged slides by covering them with poly-lysine

We have a surplus of positively charged slides (Superfrost Plus Microscope Slides) since they did not perform well with small zebrafish brain slices, which were washed off from these slides during...

01 January 1970 3,694 0 View

Weak DAPI staining after immunohistochemistry

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

01 January 1970 9,137 7 View