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Our lab use thermoscientific pH meter orion 3-star. I made 1 M sodium phosphate buffer following NEB protocols: Solution A: Dissolve 138.0 g NaH2PO4–H2O in 1 liter dH2O (pH 7.0). Solution...
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Problem: I transformed E.coli (DH10B strain), plasmid(KmR) checked by PCR and sequencing, grew them O.N., and freezed them as glycerol stock. Now, I took my glycerol stock to start again a O.N....
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