When purifying RNA with Trizol, what is the basis for selecting the centrifugation speed in the ethanol wash step?

Hi! I isolate RNA from Gram-positive bacteria using Trizol reagent (Invitrogen). I would like to know why the centrifugation speed should be reduced from 12000 g to 7500 g during the ethanol wash...

25 November 2023 8,369 3 View